Inhibitory effect of sodium valproate on human lung carcinoma SPC-A1 cell proliferation and the mechanism / 南方医科大学学报

<p><b>OBJECTIVE</b>To observe the effect of sodium valproate (VPA) on the proliferation and apoptosis of human lung carcinoma SPC-A1 cells and the underlying mechanism.</p><p><b>METHODS</b>The effect of VPA on the proliferation of SPC-A1 cells was evaluated by MTT assay and clone formation assay. Flow cytometry was used to analyze the apoptosis of the cells exposed to VPA. The changes in the expressions of Bcl-xl, Bcl-2, Mcl-1, caspase-9, and caspase-3 in the exposed cells were detected by Western blotting.</p><p><b>RESULTS</b>Incubation with VPA for 48 h resulted in a significant inhibition of SPC-A1 cell proliferation, with a IC(50) of 1.8 mmol/L. VPA treatment also inhibited cell colony formation and induced obvious cell apoptosis. Exposure to 8 mmol/L VPA for 48 h caused a percentage of early apoptotic cells of 60.44%. VPA treatment at different concentrations for 48 h obviously lowered the protein levels of Bcl-xl, Bcl-2, and Mcl-1 and induced caspase-9 and caspase-3 activation in SPC-A1 cells.</p><p><b>CONCLUSION</b>VPA can inhibit the proliferation of SPC-A1 cells by triggering mitochondrion-dependent apoptosis.</p>

The variance of serum IL-18 level and lymphocyte subpopulations in the patients with obese diabetic / 中国基层医药

Objective To observe on the variance of serum IL-18 level and lymphocyte subpopulations in the patients with obese diabetic(T2DM).Methods The diabetic obese group contained 31 cases,the non-diabetic obese group contained 33 cases,healthy normal body weight group contained 23 cases,the blood glucose(FPG),insulin (FPI),blood total cholesterol(TC),triglyceride(TG),IL-18 and the cell population of lymphocyte subpopulations CD3+and CD4+、CD8+ in peripheral blood were determined,the insulin sensitivity index were calcnlated.Results Compared with the healthy normal body weight group,the blood fat,FPG,FPI,IL-18 of T2DM patients significantly increased,there were significant statistical difference(P ＜ 0.05 or P ＜ 0.01).The ISI significantly decreased(P ＜ 0.01).Compared with the non-diabetic obese group,the blood fat and IL-18 of T2DM patients significantly increased (P＜0.05).Compared with the diabetic obese group,the subpopulations cell population of CD3+、CD4+、CD8+ in the non-diabetic obese group significantly decreased,the ratio of CD4+/CD8+ significantly increased,there were significant statistical difference(P＜0.05 or P＜0.01)in the two groups comparison.Compared with the non-diabetic obese CD4+/CD8+ significantly increased(P＜0.05).Conclusion T2DM patients should prevent the hyperinsulinemia,control blood sugar,reduce insulin resistance,sustain normal lipid metabolism,boost immune function.

Relationship between urinary ceruloplasmin and diabetic nephropathy / 中国医师进修杂志

Objective To study the relationship between urinary ceruloplasmin(CP)and diabetic nephropathy. Methods Two hundred and thirty-six patients with type 2 diabetes mellitus were divided into normal albuminuria group(DMN group, 129 cases) and micro-albuminuria group ( DMMA group, 107 cases).Eighty-one healthy people were admitted into control group. The levels of urinary CP, serum CP, urinary albumin(Alb)and urinary creatinine(Cr)were measured. Results The median of urinary CP/Cr in DMMA group (2.55 ng/mmol) and DMN group (1.18 ng/mmol )was higher than that in control group(0.92 ng/mmol )(P<0.01 ). There was no significant difference in the levels of serum CP in each group (P>0.05 ).The urinary Alb/Cr was significantly positively correlated with urinary CP/Cr (r=0.188 ,P<0.01 )in patients with type 2 diabetes mellitus. The course of diabetes mellitus was a risk factor of urinary CP/Cr and Alb/Cr increase. Conclusions The urinary CP shows increasing tendency in accord with urinary Alb in type 2 diabetes mellitus patients. Urinary CP elevates obviously and correlates with diabetic nephropathy. Urinary CP may be used to monitor the onset, progress and therapeutic effect of diabetic nephropathy.

Effects of short-term insulin pump treatment on pancreatic ?-cells in newly diagnosed type 2 diabetes mellitus / 西安交通大学学报(医学版)

Objective To explore the effects of insulin pump on pancreatic ?-cells in type 2 diabetes mellitus accompanied with hyperglycaemia.Methods We treated 46 cases of newly diagnosed type 2 diabetes mellitus with fasting blood glucose≥10.0 mmol/L by insulin pump for 2 weeks.Their blood sugar and insulin were determined before and after treatment by oral glucose tolerance test and insulin releasing test.We then calculated the function index,morning secretion of pancreatic ?-cells and insulin resistance index.The patients were followed up for 6 months and the blood glucose was monitored.Results After treatment,insulin,function index and the morning secretion were increased significantly,while the blood sugar and resistance index were decreased.In all the patients,16 could maintain ideal blood glucose only by alimentary control and exercise.Conclusion With the insulin pump in a short term,patients with type 2 diabetes mellitus can rapidly control blood glucose,obviously improve pancreatic ?-cells,and reduce insulin resistance.